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The gentle-clipped sequences that overhang the breakpoint are collapsed into a consensus sequence utilizing a several sequence alignment (MSA) provided during the seqAn library [27]. Wham applies a few filters to your consensus sequences. Breakpoints are usually not reported in circumstances in which consensus sequences are shorter than 10 bp or co
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Wham is created for paired-conclude Illumina libraries with standard insert measurements (~300bp-500bp). Wham integrates mate-pair mapping, split go through mapping, soft-clipping, alternate alignment and consensus sequence based proof to predict SV breakpoints with single-nucleotide precision. Wham generates a put together pileup (catalog of reads